Cerebrospinal Fluid Gamma-globulin in Multiple Sclerosis

Multiple Sclerosis Cerebrospinal Fluid Biomarkers

Cerebrospinal fluid (CSF) is the body fluid closest to the pathology of multiple sclerosis (MS). For many candidate biomarkers CSF is the only fluid that can be investigated. Several factors need to be standardized when sampling CSF for biomarker research: time/volume of CSF collection, sample processing/storage, and the temporal relationship of sampling to clinical or MRI markers of disease ac...

Comparison of Immunological Specificity of Gamma Globulin in the Cerebrospinal Fluid in Normal and Multiple Sclerosis Subjects.

It is generally agreed that y globulin concentration in spinal fluid is elevated in some 60% of cases of multiple sclerosis (Kabat, Freedman, Murray, and Knaub, 1950; Roboz, Hess, and Forster, 1953; Yahr, Goldensohn, and Kabat, 1954; Ivers, McKenzie, McGuckin, and Goldstein, 1961; Schapira and Park, 1961; Foster and Horn, 1962). Whilst little is known of its origin or direct pathological signif...

Cerebrospinal Fluid Proteomics of Multiple Sclerosis Patients

Elevated levels of PPAR-gamma in the cerebrospinal fluid of patients with multiple sclerosis.

Peroxisome proliferator-activated receptor gamma (PPARγ), a ligand-activated transcriptional factor involved in the regulation of glucose and lipid metabolism, has gained interest as a potential therapeutic target in multiple sclerosis (MS) due to its potent immunoregulatory properties and the therapeutic efficacy of its ligands in experimental autoimmune encephalitis (EAE). Elevated expression...

Isoelectric focusing of gamma globulins in cerebrospinal fluid from patients with multiple sclerosis.

The technique of isoelectric focusing has been adapted for rapid clinical analysis for globulins in cerebrospinal fluid with use of commercially prepared horizontal-slab acrylamide gels. The globulin fraction is concentrated by ammonium sulfate precipitation, which allows more of the relevant protein to be applied, use of a wider range of total protein concentrations, and higher resolution than...